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Developmental profile of mitochondrial glycineN-acyltransferase in human liver

Authors
Journal
The Journal of Pediatrics
0022-3476
Publisher
Elsevier
Publication Date
Volume
130
Issue
6
Identifiers
DOI: 10.1016/s0022-3476(97)70293-2
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Objective: To study the developmental profile of glycine N -acyltransferase (GAT) in the livers of children of various ages and to compare the total and specific GAT activity with that of the adult control subjects. Methods: We measured the specific and the total mitochondrial activity of GAT in liver samples taken from 13 children 4 hours to 11 years of age. The samples were compared with those of control adults aged 24 to 40 years. Samples, either from liver-transplant donors or from autopsy, from those who died of a disorder not related to the liver, were obtained between 6 and 36 hours after death. Results: At 4 hours after birth, very low specific activity and the total liver mitochondrial activity were observed (0.19 μmol/min per milligram protein and 210 mmol/min), with a steady increase up to age 7 months (2.51 μmol/min per milligram protein and 812 mmol/min). The mean specific and total GAT activity in children (n = 5) aged 18 months to 11 years was 6.38 ± 0.13 and 1389 ± 43 and in control adults aged 24 to 40 years (n = 3) was 6.5 ± 0.3 and 1461 ± 71 μmol/min per milligram protein and μmol/min, respectively. These specific and total GAT activity values from children aged 18 months to 11 years were not statistically significant (by analysis of variance and Mann-Whitney test) in comparison with the corresponding activity values from the adult control subjects. Conclusions: Our results indicate that up to age 7 months, children have only 5% to 40% of liver GAT-specific activity, whereas the peak activity is achieved at 18 months and remains constant until age 40 years. The delayed development of GAT in children may thus compromise the detoxification of various drugs and xenobiotics. (J Pediatr 1997;130:1003-7)

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