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Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived fromS. pneumoniaetype 14 capsular polysaccharide by a chemoenzymatic method1 This is NRCC publication No. 39584.1

Authors
Journal
Carbohydrate Research
0008-6215
Publisher
Elsevier
Publication Date
Volume
309
Issue
3
Identifiers
DOI: 10.1016/s0008-6215(98)00134-7
Keywords
  • Type Iii Group Bstreptococcus
  • Capsular Polysaccharide
  • Oligosaccharides

Abstract

Abstract Partial N-deacetylation of the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3- O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis.

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