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16S rRNA gene sequencing reveals the relationship between gut microbiota and ovarian development in the swimming crab Portunus trituberculatus.

Authors
  • Wei, Hongling1
  • Li, Xing2
  • Tang, Lei3
  • Yao, Hongzhi4
  • Ren, Zhiming5
  • Wang, Chunlin6
  • Mu, Changkao7
  • Shi, Ce8
  • Wang, Huan9
  • 1 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 2 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 3 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 4 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 5 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 6 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 7 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 8 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
  • 9 School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China; Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, Zhejiang, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Chemosphere
Publication Date
Sep 01, 2020
Volume
254
Pages
126891–126891
Identifiers
DOI: 10.1016/j.chemosphere.2020.126891
PMID: 32957291
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Gut microbiota executes many beneficial functions. In this study, the relationship between gut microbiota and ovarian development in the swimming crab P. trituberculatus was explored for the first time. A total of 28 phyla and 422 genera were identified across all samples. However, 105 differential operational taxonomic units, and four differential phyla (Gemmatimonadetes, Actinobacteria, Firmicutes, Marinimicrobia_(SAR406_clade)) were identified. At the genus level, 42 differential genera were identified and 144 bacterial indicators were identified. A key finding was that the relative abundance of 139 indicator bacteria detected in the anisomycin-2 mg/kg group (AK group) was higher than that of blank group (BK group), control group (CK group), SP600125-15 mg/kg group (SK group). In addition, the relative abundance of three indicator bacteria (OTU_236, OTU_1395, OTU_552) detected in the SK group was higher than that of the BK, CK and AK groups. It was also found that the relative abundance of 20 differential genera (Methyloversatilis, Coprococcus_1, Erysipelotrichaceae_UCG_003, Rikenella, Corynebacterium, Ruminiclostridium, Fusicatenibacter, [Eubacterium]_ruminantium_group, Rikenellaceae_RC9_gut_group, Bifidobacterium, Lachnospiraceae_NK4A136_group, Ruminococcaceae_UCG_014, Christensenellaceae_R_7_group, uncultured_Bacteroidales_bacterium, Coprococcus_2, Desulfovibrio, Aggregatibacter, Ambiguous_taxa, Alloprevotella and Ruminococcaceae_NK4A214_group) in the SK, BK, CK, and AK group samples were increasing. These differential genera may reveal the relationship between gut microbial communities and ovarian development in P. trituberculatus after injection with the JNK pathway inhibitor SP600125 or the activator anisomycin. In summary, this study provides a new understanding into the relationship between gut microbiota and ovarian development in response to stimulation with inhibitor or activator. Copyright © 2020 Elsevier Ltd. All rights reserved.

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