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Detection of receptors for the Fc region of IgG on streptococci

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
59
Issue
1
Identifiers
DOI: 10.1016/0022-1759(83)90148-5
Keywords
  • Streptococci
  • Fc-Receptors
  • Protein A
Disciplines
  • Biology

Abstract

Abstract A semi-quantitative absorption test to measure Fc-reactive proteins on the surface of streptococci is described. The ability of bacteria to remove intact IgG in the presence of an equimolar amount of F(ab′) 2 fragments was used to identify streptococci with Fc-reactive proteins on their surface. This method was found to be more objective, reproducible and quantitative than the hemagglutination and 125I-labeled IgG binding methods currently in use. Methods for detection of secreted Fc-reactive materials with protein A-like reactivity are also described.

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