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Induction of unspliced c-fos messenger RNA in rodent brain by kainic acid and lipopolysaccharide

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
305
Issue
1
Identifiers
DOI: 10.1016/s0304-3940(01)01793-1
Keywords
  • Transcription Factors
  • Primary Cell Culture
  • Kainic Acid
  • Gene Regulation
  • Phosphorylation
  • Seizure
Disciplines
  • Biology

Abstract

Abstract The c-fos transcriptional factor forms an activator protein-1 (AP-1) complex with proteins from the Jun family, which plays an important role in the central nervous system. The responses of AP-1 transcriptional factors induced by kainic acid (KA) treatment have been well studied, although the transcriptional regulation of these KA-induced factors has not been clearly characterized. To investigate the role of different stimuli in controlling of the splicing of c-fos mRNA, we performed reverse transcriptional polymerase chain reaction. The results showed that spliced and unspliced c-fos is present in rat brain following KA treatment and in lipopolysaccharide (LPS)-treated primary mouse cortical brain cell cultures. Furthermore, tyrosine kinase and protein phosphatase inhibitors alter the preponderance of c-fos transcripts following LPS treatment.

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