Abstract Fasted intact rats were injected with glycine-1-C 14 and glucose-1-C 14, and the effects of glucagon and epinephrine on incorporation of the C 14 into liver glycogen and expired carbon dioxide were studied. 1. 1. The rate of incorporation of C 14 from glycine into liver glycogen was increased by a previous injection of glucagon, and decreased by a previous injection of epinephrine. 2. 2. The rate of incorporation of glycine-1-C 14 into glycogen was decreased, while the incorporation into CO 2 was increased by concurrent injections of glucagon. 3. 3. The incorporation of C 14 from glucose into liver glycogen was increased by a previous injection of glucagon. 4. 4. Glucagon appears to cause a “rebound” accumulation of liver glycogen comparable to that produced by epinephrine.