We investigated the effects of different doses of honey (H) and testosterone (T) on sperm count and reproductive hormones in male albino rats. Thirty-five male albino rats were randomly divided in a blinded fashion into 7 groups of 5 rats each. Group 1 (control) was given 0.2 ml of distilled water. Groups 2, 3 and 4 were given 100 mg/kg, 200 mg/kg and 400 mg/kg of H orally respectively. Groups 5, 6 and 7 were given 2.5 mg/kg, 5 mg/kg and 7.5 mg/kg of T intraperitoneally respectively. All doses of H significantly (P<0.05) increased sperm count in rats, while all the doses of T significantly reduced sperm count in rats. Plasma T was increased and FSH was reduced (P<0.001) by all the three doses of H. On the contrary, LH was significantly reduced (P<0.05) by 100 mg/kg and 200 mg/kg of H but not by 400 mg/kg of H. All the three doses of T reduced the plasma T and LH in rats. Lastly, 5 mg/kg and 7.5 mg/kg but not 2.5 mg/kg of T reduced FSH in rats. The results suggest that honey enhanced sperm count in rat by increasing testosterone production.Keywords: Gonadotropins, Honey, Sperm count, Testosterone.