Abstract A model of the 3-dimensional structure of the antennal lobes in the brain of the worker honey bee, Apis mellifera L. (Hymenoptera : Apidae), has been constructed using retrograde cellular marking and computer reconstruction techniques. The model provides a pictorial representation of the spatial organization of structures within the antennal lobe neuropil. In addition, an atlas is provided to aid the identification of invariant morphological landmarks within the antennal lobe, some of which have been described previously. Readily identifiable invariant glomeruli are described in detail, and a hitherto unreported afferent tract of the antennal nerve, the tract “T2-2”, is also identified. Precise descriptions of the arborizations patterns of several deutocerebral interneurones stained intracellularly with cobalt serve to illustrate the advantages of using the atlas and 3-dimensional representations of the antennal lobe represented in this paper.