Affordable Access

Publisher Website

Study of the Bohr groups of bovine hemoglobin

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Protein Structure
0005-2795
Publisher
Elsevier
Publication Date
Volume
188
Issue
2
Identifiers
DOI: 10.1016/0005-2795(69)90067-1

Abstract

Abstract The Bohr effect of bovine hemoglobin and of bovine hemoglobin in which protonic groups were substituted by reaction with FDNB was studied by means of normal, differential and difference titration curves. A number of results were obtained by comparison of calculated curves with the experimental curves. This study revealed the following facts. 1. 1. The presence of the normal and acid Bohr groups is easily seen from the differential titration curves of Hb and HbO 2 respectively. 2. 2. An analysis of these curves shows that only 18 of the 32 histidine residues are titratable and that 4 of these 18 residues have an abnormally high pK of about 8 in Hb as well as in HbO 2. 3. 3. From a comparison of the differential and difference titration curves of substituted hemoglobin with those of unsubstituted hemoglobin, it is concluded that 2 of the 4 α-amino groups are alkaline Bohr groups.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Study of the Bohr groups of bovine hemoglobin.

on Biochimica et Biophysica Acta 1969

Study of the Bohr effect in hemoglobin intermediat...

on G Protein Coupled Receptors -... 1998

Changes in the Bohr effect due to pyridoxylation o...

on Biochimica et Biophysica Acta Nov 09, 1984

Changes in the Bohr effect due to pyridoxylation o...

on Biochimica et Biophysica Acta... Jan 01, 1984
More articles like this..