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IN VITRO CYTOTOXICITY BY A NONTHYMUS-PROCESSED LYMPHOCYTE POPULATION WITH SPECIFICITY FOR A VIRALLY DETERMINED TUMOR CELL SURFACE ANTIGEN

Authors
Journal
Journal of Experimental Medicine
0022-1007
Publisher
The Rockefeller University Press
Publication Date
Keywords
  • Article

Abstract

1072.tif IN VITRO cY ' rOTOXIC ITY BY A NONTHYMUS-PROCESSED LYMPHOCYTE POPULATION WITH SPECIF IC ITY FOR A VIRALLY DETERMINED TUMOR CELL SURFACE ANTIGEN* BY ~E. W. LAMON,:~ H. M. SKURZAK,§ E. KLEIN, AN'D H. WIGZELL (From the Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden) (Received for publication 19 June 1972) A lymphocyte population may be subdivided into developmental and functional categories. The bone marrow-derived (B) lymphocytes have a high concentration of surface immunoglobulin (Ig) 1 (1-3) and provide the precursors of antibody-secreting cells (4). The thymus-processed (T) lymphocytes, on the other hand, have a very low, if at all detectable surface Ig concentration (1.-3) and have the capability to become cytotoxic to target ceils bearing histocompatibility antigens against which the T cells have been sensitized (5, 6). It seems likely, however, that cells other than T cells can be killer cells for target cells. For example, in one system using antibody-coated target cells the cell-mediated lysis appears to be a non-T cell function (7, 8). With the development of methods for isolation of B- or T cell-deficient populations it has been possible to delineate the functions of these cells and some of their inter- relationships. Cytotoxicity studies using immune B- and T cell-deficient populations have been performed within the H-2 system (5, 6) demonstrating T cells to be the dominating killer cells in these tests. With tumor-specific and/or virally determined antigens in syngeneic hosts, T vs. B or non-T cytotoxicity in vitro have not been de- fined. Using Moloney sarcoma virus (MSV)-induced tumors in BALB/c mice it has been demonstrated that, from animals in which tumors have regressed, lymphocytes are cytotoxic against cells carrying the Moloney leukemia virus (MLV)-determined surface antigen (9). In the present investigation lympho- cyte cytotoxicity was examined against target cells possessing the MLV surface

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