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Investigation of the carboxyl ionization of β-lactoglobulin by differential infrared spectroscopy

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
85
Issue
2
Identifiers
DOI: 10.1016/0003-9861(59)90508-9
Disciplines
  • Biology
  • Physics

Abstract

Abstract A differential infrared study has been carried out as a function of pH on β-lactoglobulin in the region of carboxyl absorption. The observations of Tanford and co-workers that this protein contains two carboxyl groups that remain un-ionized up to pH 6.5 has been confirmed.

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