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Separation of radioiodinated angiotensins by chromatofocusing in minicolumns

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
138
Issue
2
Identifiers
DOI: 10.1016/0003-2697(84)90812-1
Keywords
  • Angiotensins
  • Chromatofocusing
  • Radioiodine Labeling
  • Hormone
  • Peptides
  • Chromatography

Abstract

Abstract Angiotensins I, II, and III (AI, AII, AIII) and Saralasin (Sar 1-Ala 8-AII) were labeled with 125I and separated from the nonlabeled forms on minicolumns (a Pasteur pipet) of chromatofocusing medium. At low ionic strength, 125I-labeled angiotensins could be eluted with Polybuffer or a piperazine-histidine buffer at their approximate isoelectric points, while nonlabeled angiotensins remained adsorbed to the column and required 1 mol · liter −1 NaCl for elution. The 125I-labeled angiotensins prepared by this method were bound by antibodies (AI) and adrenal receptors (AII, Saralasin) to an extent similar to angiotensins prepared by DEAE-Sephadex A-25 chromatography. This new method of preparing radioiodinated angiotensins is rapid (15 min), inexpensive, and requires no fraction-collecting equipment.

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