As is the case for the whole of Croatia, most of the data on the spider faunamof Dalmatia dates back to the early 20th century. However, some recent works on the spider fauna of Dalmatia have been published by Rucner & Rucner (1995), Dobroruka (2004) and Majer et al. (2008). Recently, a considerable contribution to the knowledge on spider fauna of Dalmatia was made by the Association for Biological Research – BIOM through their research into central Dalmatian nature-protected areas. In order to increase the knowledge on the spider fauna of Dalmatia, the Spider Section of the Biology Students Association BIUS, conducted research into the spider fauna of Kornati National Park in 2009. The research was performed as part of the biology research camp “Kornati 2009”, organized by BIUS. The Spider Section spent a total of 12 days in the field, from 14th to 21st of May and from 26th of September to 1st of October 2009. The research covered Kornat Island and several neighbouring islands, which are a part of the National Park: Gustac, Piškera, Lavsa, Levernaka, Mana and Velika Smokvica. Sampling sites were selected according to habitat types, i.e. olive groves, rocky pastures, dry meadows and pine forests. The aim was to include as many different habitats as possible, in order to collect the maximum possible spider taxa. Forty-two different taxa were identified: 31 to the species level and 11 to the genus, divided into 19 families. The remaining 6 taxa were represented with juvenile specimens, which could not be identified to the species level, but probably belong to the identified species. The families Araneidae, Lycosidae, Salticidae, Theridiidae were represented with the highest number of species. We found 2 new species for the Croatia araneofauna.