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Detection of Epstein-Barr virus (EBV) DNA sequences using in situ hybridization

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
0005-2787
Publisher
Elsevier
Publication Date
Volume
519
Issue
1
Identifiers
DOI: 10.1016/0005-2787(78)90061-8
Disciplines
  • Design

Abstract

Abstract In situ hybridization was used to detect Epstein-Barr virus (EBV) DNA sequences under conditions where the virus DNA is replicating spontaneously and where it is induced to do so following superinfection. The in situ reaction itself is influenced by several parameters, analogous to conventional nucleic acid hybridization, consideration of which should help to optimize the designing of in situ hybridization reactions in general. Both EBV complementary RNA (cRNA) and EBV DNA synthesized in vitro can efficiently detect the virus DNA sequences in situ. The findings presented here can therefore be utilized in both the study of EBV-cell interactions and, more generally, in studies using in situ hybridization as a general approach.

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