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High-performance liquid chromatography of non-polar retinoid isomers

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
881
Identifiers
DOI: 10.1016/s0021-9673(00)00232-6
Keywords
  • Retinoids
  • Vitamins

Abstract

Abstract Normal-phase high-performance liquid chromatograms of retinal, retinol and retinyl palmitate isomers using n-heptane– tert.-butyl methyl ether as mobile phase are presented. Methods for the synthesis of various isomers of these retinoids are described. This enables one to produce standard chromatograms and to select various isomers for cochromatography and the identification of the various peaks under study. Assignment of the peaks is based on chromatograms published previously in this journal. For the main isomers it is possible to get baseline separation of the commonly occurring isomeric forms in a reasonably short analysis time.

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