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Increased lymphocyticNa + H +exchange activity after hemodialysis: Evidence for an endogenous inhibitor ofNa + H +exchange in patients with end-stage renal failure

Authors
Journal
Life Sciences
0024-3205
Publisher
Elsevier
Publication Date
Volume
59
Issue
18
Identifiers
DOI: 10.1016/0024-3205(96)00485-7
Keywords
  • Na + Hexchange
  • Lymphocytes
  • End-Stage Renal Failure

Abstract

Abstract The Na + H + exchange antiport activity was measured in lymphocytes from 16 patients with end-stage renal failure pre- and postdialysis. In addition the effect of the patients' plasma on lymphocytes from healthy subjects was tested. Resting pH (pH i) was not significantly different in lymphocytes pre- and postdialysis. On the other hand, the Na + H + exchange activity was significantly lower in lymphocytes before hemodialysis (6.22 ± 0.73 ×10 −3 pH i / s) than after hemodialysis (9.32 ± 1.58 × 10 −3 pH i s ; n = 16; p<0.05). The buffer capacity was not significantly different before and after hemodialysis. The incubation of lymphocytes from healthy control subjects with plasma from patients with end-stage chronic renal failure significantly reduced the lymphocytic Na + H + exchange activity. The addition of ultrafiltrate also significantly reduced the Na + H + exchange activity in lymphocytes from healthy control subjects. The study indicates the existence of an endogenous inhibitor of the Na + H + exchange that is accumulated in plasma from patients with end-stage chronic renal failure

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