Alignment of the conserved region ofLeishmania kDNA minicircles showing the highly conserved sequence blocks (bold). Yurchenko V Y et al. J. Clin. Microbiol. 1999;37:1656-1657 Alignment of the conserved region ofLeishmania kDNA minicircles showing the highly conserved sequence blocks (bold). The primers used in the nested PCR are shown. The reverse primers have been reversed and complemented. The alignment shows that the regions selected for the primers are highly conserved across the wide range of species used. It is therefore assumed that they will also be conserved across most minicircle classes in a given strain. The Genbank accession numbers of the strains are as follows:L. braziliensis, M87315; L. guyanensis, M87316;L. infantum 1, Z32847; L. infantum 2, Z32846;L. infantum 3, Z35501; L. peruviana, M87317;L. tropica, Z32843; L. infantum 4, Z35269;L. infantum 5, Z35270; L. infantum 6, Z35272;L. infantum 7, Z35273; L. infantum 8, Z35274;L. infantum 9, Z35292; L. infantum 10, Z35501-2;L. infantum 11, Z35501.