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An Application of Molecular Genotyping in Mice

Authors
Journal
Biological Procedures Online
1480-9222
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
5
Issue
1
Identifiers
DOI: 10.1251/bpo53
Keywords
  • Research Article
Disciplines
  • Biology
  • Medicine

Abstract

Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction. Different strains of mice have variable CA repeat lengths and PCR based methods can be used to identify them, thus allowing for specific genotypes to be assigned. Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied. We studied chromosomal segregation in relation to nondisjunction in early-gestation mouse embryos using molecular genotyping. Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

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