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Evidence for mannosidase activities inStreptococcus oraliswhen grown on glycoproteins as carbohydrate source

Authors
Journal
Carbohydrate Research
0008-6215
Publisher
Elsevier
Publication Date
Volume
312
Issue
3
Identifiers
DOI: 10.1016/s0008-6215(98)00246-8
Keywords
  • Mannosidase
  • Streptococcus
  • Ribonuclease B
  • Maldi
Disciplines
  • Biology

Abstract

Abstract Streptococcus oralis when cultured using ribonuclease B as the sole source of carbohydrate, selectively uses the sugars of the Man 5 glycoform as shown by HPAEC and MALDI–TOF mass spectrometric analyses. The organism is able to do this by producing novel α-(1⃗3), α-(1⃗6) and β-(1⃗4) mannosidase activities and these act in a concerted manner in what appears as a single-step process. The selective utilisation of Man 5 is explained by the absence of an α-(1⃗2) mannosidase which is required to initiate breakdown of the glycan chains present in the other glycoforms which are components of the glycoprotein.

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