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Antiserum raised against gyrase A ofAcholeplasma laidlawiireacts with phytoplasma proteins

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Volume
206
Issue
2
Identifiers
DOI: 10.1016/s0378-1097(01)00533-x
Keywords
  • Gyrase A Antibody
  • Acholeplasma
  • Phytoplasma
  • Mollicute
Disciplines
  • Biology

Abstract

Abstract Part of the gyrase A gene ( gyrA) of Acholeplasma laidlawii was cloned and incorporated directly downstream from a 6×His tag segment of the pQE expression vector. The 23-kDa fusion protein was expressed as a 6×His-tagged protein in Escherichia coli. The fusion protein was purified and used as an antigen for rabbit immunization. Western immunoblot analysis revealed that the antiserum raised against the gyrase A fragment had a specific affinity for a 108-kDa protein of A. laidlawii and cross-reacted with a 107.5-kDa protein of Acholeplasma axanthum, a 107-kDa protein of Acholeplasma granularum, and 95–97-kDa proteins of several phytoplasma-infected plants. The antiserum could also detect phytoplasmas in infected plant sap. These results demonstrate that the gyrase A protein (GyrA) of A. laidlawii shares antigenicity with the GyrA of other Acholeplasma species and also with those of phytoplasmas including some from a few groups with unrelated 16S rRNAs.

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