Affordable Access

Publisher Website

A fluorometric assay for dihydrofolate reductase

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
83
Issue
1
Identifiers
DOI: 10.1016/0003-2697(77)90504-8
Disciplines
  • Biology

Abstract

Abstract A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

A fluorometric assay for dihydrofolate reductase.

on Analytical Biochemistry November 1977

A novel fluorometric assay for quantitative analys...

on Journal of Biochemical and Bio... September 1987

A sensitive high-performance liquid chromatographi...

on Analytical Biochemistry Aug 01, 1984
More articles like this..