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Pulsed Ultraviolet Light Reduces Immunoglobulin E Binding to Atlantic White Shrimp (Litopenaeus setiferus) Extract

Authors
Publication Date
Keywords
  • Allergen
  • Allergy
  • Shrimp
  • Pulsed Ultraviolet Light
  • Puv
  • Tropomyosin
  • Ige Antibodies
Disciplines
  • Biology
  • Physics

Abstract

Pulsed Ultraviolet Light Reduces Immunoglobulin E Binding to Atlantic White Shrimp (Litopenaeus setiferus) Extract Int. J. Environ. Res. Public Health 2011, 8, 2569-2583; doi:10.3390/ijerph8072569 International Journal of Environmental Research and Public Health ISSN 1660-4601 www.mdpi.com/journal/ijerph Article Pulsed Ultraviolet Light Reduces Immunoglobulin E Binding to Atlantic White Shrimp (Litopenaeus setiferus) Extract Sandra Shriver 1 , Wade Yang 1, *, Si-Yin Chung 2 and Susan Percival 1 1 Department of Food Science & Human Nutrition, University of Florida, P.O. Box 110370, 359 FSHN Bldg. Newell Drive, Gainesville, FL 32611, USA; E-Mails: [email protected] (S.S.); [email protected] (S.P.) 2 Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd., Bldg 001 SRRC, New Orleans, LA 70124, USA; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +1-352-392-1991 ext. 507; Fax: +1-352-392-9467. Received: 18 May 2011 / Accepted: 19 June 2011 / Published: 24 June 2011 Abstract: Pulsed ultraviolet light (PUV), a novel food processing and preservation technology, has been shown to reduce allergen levels in peanut and soybean samples. In this study, the efficacy of using PUV to reduce the reactivity of the major shrimp allergen, tropomyosin (36-kDa), and to attenuate immunoglobulin E (IgE) binding to shrimp extract was examined. Atlantic white shrimp (Litopenaeus setiferus) extract was treated with PUV (3 pulses/s, 10 cm from light source) for 4 min. Tropomyosin was compared in the untreated, boiled, PUV-treated and [boiled+PUV]-treated samples, and changes in the tropomyosin levels were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE binding of the treated extract was analyzed via immunoblot and enzyme-linked

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