Affordable Access

Publisher Website

Characterization of Na+-independent Mg2+efflux from erythrocytes

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
271
Identifiers
DOI: 10.1016/0014-5793(90)80394-x
Keywords
  • Magnesium
  • Chloride
  • Efflux
  • Amiloride
  • Camp
  • Erythrocyte

Abstract

Abstract Na +-independent Mg 2+ efflux from Mg 2+-loaded human, rat and chicken erythrocytes was reduced by extracellular Cl −. Na +-independent Mg 2+ efflux at low extracellular Cl − concentration (sucrose medium) was inhibited by SITS and was nearly insensitive to SITS in 150 mM choline Cl medium. The inhibition of Mg 2+ efflux by extracellular Cl − and DIDS could be overcome by the lipophilic permeant tetraphenylphosphonium cation. Na +-independent Mg 2+ efflux from human and rat erythrocytes in sucrose and choline Cl medium was inhibited by cAMP and by amiloride and amiloride analogues. The results indicate that Na +-independent Mg 2+ efflux in high Cl − medium is performed by a similar or the same Mg 2+ efflux system, operating in sucrose medium in which the efflux of Mg 2+ is accompanied by the efflux of Cl − for charge compensation.

There are no comments yet on this publication. Be the first to share your thoughts.