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Production of neo-fructooligosaccharides using free-whole-cell biotransformation byXanthophyllomyces dendrorhous

Authors
Journal
Bioresource Technology
0960-8524
Publisher
Elsevier
Publication Date
Volume
101
Issue
19
Identifiers
DOI: 10.1016/j.biortech.2010.04.026
Keywords
  • β-Fructofuranosidase
  • Xanthophyllomyces Dendrorhous
  • Neo-Fructooligosaccharides
  • Free-Whole-Cell
  • Biotransformation

Abstract

Abstract The effects of production parameters on the biotransformation of sucrose were investigated to enhance the yield of neo-FOS by Xanthophyllomyce dendrorhous cells. Cells showed optimal β-fructofuranosidase activity at neutral pH condition and the yield of neo-FOS showed no significant differences between buffer and buffer-free systems. Cell concentration negatively affected the maximum neo-FOS yield. Sucrose concentration positively increased the maximum yield of neo-FOS. Elevating the reaction temperature to 30 °C, the neo-FOS productivity increased 1.85-fold compared with that at 20 °C. Meanwhile, cell age of 32 h enabled the biotransformation of sucrose more efficiently. In addition, free cells exhibited a higher productivity over immobilized cells. The maximum neo-FOS concentration finally reached 227.72 g/l from 400 g/l sucrose under the optimal conditions.

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