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An enzyme-linked double antibody immunoassay to measure murine immunoglobulins — Its application to determine the specific activity of radiolabeled monoclonal antibodies

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
82
Issue
2
Identifiers
DOI: 10.1016/0022-1759(85)90355-2
Keywords
  • Monoclonal Antibodies
  • Enzyme Immunoassay

Abstract

Abstract A sensitive, simple and reproducible biotin-avidin amplified double antibody immunoassay to quantitate low concentrations of mouse immunoglobulins is described. The assay is a useful technique to measure trace levels of murine monoclonal antibodies in culture supernatants of hybridoma cells metabolically labeled with radioactive isotopes. A combination of radioactive counting and measurement of the absorbance of a peroxidase catalyzed reaction permits accurate determination of the specific radioactivity of labeled monoclonal antibodies.

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