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Protein synthesis in cell-free preparations fromMusca domesticaL.

Authors
Journal
Comparative Biochemistry and Physiology
0010-406X
Publisher
Elsevier
Publication Date
Volume
21
Issue
3
Identifiers
DOI: 10.1016/0010-406x(67)90444-6
Disciplines
  • Biology

Abstract

Abstract 1. 1. Protein synthesis in a cell-free system from Musca domestica L. has been shown to occur by the pathway involving amino acyl-adenylates and amino acyl-soluble ribonucleic acid as intermediates. 2. 2. The system requires an ATP-generating system, a divalent cation and a monovalent cation, guanosine-5′-triphosphate and microsomes or ribosomes and pH 5 enzymes for optimal activity. 3. 3. The requirement of guanosine-5′-triphosphate is not evidence in larval specimens. 4. 4. An insecticide-susceptible strain did not show a dependence on the pH 5 fraction, suggesting that the corresponding enzymes may be tightly bound to ribosomes. 5. 5. Phenylthiourea is required for optimal activity in pupal preparations, but its effect is not significant in other developmental stages.

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