Abstract A multi-channel analyzer was used to evaluate biogas potential of sugarcane bagasse (SCB). The Automatic Methane Potential Test System contained fifteen parallel reactors and the same number of gas flow meters attached to the acquisition system. The set of reactors – gas flow meters gave reproducible results during anaerobic digestion of chemically defined carbon source and the units were used to evaluate the biomethane potential of SCB after different pretreatments, such as treatment with water, acid, acid followed by enzymatic treatment and acid followed by treatment with inactive enzymes. Combined pretreatment with 2% sulphuric acid and enzymatic hydrolysis (3.5% enzymes) resulted in conversion of 79% to monomeric sugars present in SCB. SCB treated with acid followed by enzymatic hydrolysis achieved the methane yield of 200NL per kg VSadded. Enzymatic saccharification of acid pretreated SCB resulted in increase of methane yield by 16±5% compared to that from acid treated SCB.