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Efficient Serotype-Dependent Release of Functional Vector into the Culture Medium During Adeno-Associated Virus Manufacturing

Authors
Journal
Human Gene Therapy
1043-0342
Publisher
Mary Ann Liebert
Publication Date
Volume
21
Issue
10
Identifiers
DOI: 10.1089/hum.2010.107
Keywords
  • Research Articles

Abstract

Vandenberghe and colleagues demonstrate that in contrast to AAV2, most other AAV serotypes are primarily released into the media of calcium phosphate-transfected production cultures and not retained in the cell lysate. Based on this observation, the authors develop a scalable method for isolating functional vector particles from culture medium. This finding should help to streamline the production process for both laboratory and clinical applications of AAV.

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