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Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
334
Issue
1
Identifiers
DOI: 10.1016/0014-5793(93)81684-r
Keywords
  • Bradykinin
  • Kallidin
  • Kininogen
  • Kallikrein
  • Blood Pressure
  • Trout
Disciplines
  • Medicine

Abstract

Abstract Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe 6→Trp and Phe 9→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein—kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

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