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Allozyme Electrophoretic Methods for Analysing Genetic Variation in Giant Clams (Tridacnidae)



The Australian Centre for International Agricultural Research (ACIAR) was established inJune 1982 by an Act of the Australian Parliament. Its mandate is to help identify agricul- tural problems in developing countries and to commission collaborative research between Australia and developing country researchers in fields where Australia has a special research competence. Where trade names are used this constitutes neither endorsement of nor discrimination against any product by the Centre. ACIAR TECHNICAL REPORTS SERIES This series of publications contains technical information resulting from ACIAR-supported programs, projects, and workshops (for which proceedings are not being published), reports on Centre-supported fact-finding studies, or reports on other useful topics resulting from ACIAR activities. Publications in the series are distributed internationally to a selected audience. Australian Centre for International Agricultural Research, GPO Box 1571, Canberra, ACT 2601. Benne,j.A.H., Williams, S.T., and Macaranas,j.M. 1993. Allozyme electrophoretic methods for analysing genetic variation in giant clams (Tridacnidae). ACIAR Technical Reports No. 23,48p. ISBN I 863200746 Typeset and laid out by Arawang Information Bureau Pty Ltd, Canberra. Printed by Goanna Print Pty Ltd, Canberra. Allozyme Electrophoretic Methods for Analysing Genetic Variation in Giant Clams (Tridacnidae) J.A.H. Benzie s.T. Williams J.M. Macaranas Australian Centre for International Agricultural Research Canberra 1993 Contents Introduction General strategy Sample collection and storage Initial screening Survey collections Biopsy method Sample storage Protein stability Time at ambient temperature before freezing Freeze temperature Effect of freezing and thawing samples Sample freshness Electrophoresis Sample preparation for electrophoresis Starch gel electrophoresis Cellulose acetate (Cellogel) electrophoresis Scoring Enzyme surveys Enzy

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