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Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
463
Identifiers
DOI: 10.1016/s0014-5793(99)01599-9
Keywords
  • Bovine Rantes
  • Ovary
  • Macrophage
  • Eosinophil
Disciplines
  • Biology

Abstract

Abstract RANTES may be one of the chemoattractants involved in stimulating eosinophils and macrophages to migrate selectively into bovine dominant follicles and into developing corpora lutea. We sequenced a 736 bp fragment of the bovine RANTES mRNA encoding the complete protein and defined the ovarian source of RANTES mRNA. As demonstrated by competitive RT-PCR, follicle-derived macrophages showed a 100–1000 times higher RANTES mRNA level compared to unpurified granulosa cells or follicle-derived fibroblasts. By means of in situ hybridization, RANTES mRNA positive macrophages were located in the former thecal layer of the developing corpora lutea.

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