Affordable Access

Publisher Website

Optimization for quantitative determination of four flavonoids inEpimediumby capillary zone electrophoresis coupled with diode array detection using central composite design

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1103
Issue
2
Identifiers
DOI: 10.1016/j.chroma.2005.11.036
Keywords
  • Capillary Zone Electrophoresis
  • Quantitative Determination
  • Central Composite Design
  • Flavonoids
  • Epimedium
Disciplines
  • Design
  • Medicine

Abstract

Abstract Herba Epimedii (family Berberidaceae), Ying-Yang-Huo in Chinese, is a famous Chinese herbal medicine. Flavonoids are thought to be the major active components in it. A capillary zone electrophoresis (CZE) separation were developed for simultaneous determination of four flavonoids including icariin, epimedin A, epimedin B and epimedin C in Epimedium. The effects of the experimental variables on CZE had been optimized by using central composite design (CCD). The best separation of four flavonoids could be obtained using 50 mM borate buffer (pH 10.0) containing 22% acetontrile as modifier, while separation voltage was 15 kV and temperature was at 25 °C. The method developed is accurate, simple and reproducible, which could be used for quality control of Epimedium and its medical preparations.

There are no comments yet on this publication. Be the first to share your thoughts.