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Production of 6-pentyl-α-pyrone withTrichoderma atrovirideand its mutant in a novel extractive liquid-surface immobilization (Ext-LSI) system

Authors
Journal
Process Biochemistry
1359-5113
Publisher
Elsevier
Publication Date
Volume
44
Issue
6
Identifiers
DOI: 10.1016/j.procbio.2009.01.017
Keywords
  • Liquid-Surface Cultivation
  • Extractive Fermentation
  • Trichoderma
  • 6-Pentyl-α-Pyrone
  • Secondary Metabolite

Abstract

Abstract A novel extractive fermentation procedure, tentatively named an extractive liquid-surface immobilization (Ext-LSI) system, for the production of water-insoluble secondary metabolites with fungi was developed. The system using a unique polymeric micro-material, a ballooned polyacrylonitrile microshpere (MS), was applied to the production of 6-pentyl-α-pyrone (6PP), a fungicidal secondary metabolite, with a newly isolated Trichoderma atroviride AG2755-5 and its nitrosoguanidine (NTG)-mutant. The resulted mutant AG2755-5NM398 was immobilized on the surface of a liquid medium by using the MS. Following the formation of a fungus-MS mat, low toxic hydrophobic solvent, dimethyl silicone oil, was added onto the fungus-MS mat. The optimum carbon and nitrogen sources were fructose and malt extract, respectively. The higher the initial medium pH, the more the 6PP-accumulation was observed. The best MS and extractive organic solvent were MFL-80SDE (non-coated type) and KF-96L-1CS (dimethyl silicone oil). Thus, produced 6PP was spontaneously extracted from cells to the organic phase to reach 7.1 g L −1 of the accumulation in the organic phase.

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