Abstract Automated analysis and recognition of cell-nuclear phases using fluorescence microscopy images play an important role for high-content screening. A major task of automated imaging based high-content screening is to segment and reconstruct each cell from the touching cell images. In this paper we present new useful method for recognizing morphological structural models of touching cells, detecting segmentation points, determining the number of segmented cells in touching cell image, finding the related data of segmented cell arcs and reconstructing segmented cells. The conceptual frameworks are based on the morphological structures where a series of structural points and their morphological relationships are established. Experiment results have shown the efficient application of the new method for analysis and recognition of touching cell images of high-content screening.