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[50] Copy number amplification of yeast artificial chromosomes

Elsevier Science & Technology
DOI: 10.1016/0076-6879(92)16052-l
  • Section Iv. Vectors For Cloning Genes


Publisher Summary This chapter describes specific methods for generating yeast artificial chromosome (YAC) clones and for amplifying the artificial chromosomes they contain. Yeast artificial chromosome (YAC) cloning is a useful method for propagating very large DNA segments, from 100 kilobases (kb) to over 1 megabase (Mb) in size. However, artificial chromosomes are normally present in yeast at only one copy per haploid genome, hence, a number of routine applications, (such as colony-screening and preparative-scale DNA isolation) are either inconvenient to perform or are plagued with problems of low sensitivity. This chapter discusses the system that permits copy number amplification of YACs by introducing certain cis-acting elements into the cloning vector, pCGS966.

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