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Thin-layer chromatography of nucleotides, nucleosides and nucleic acid bases

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
0005-2787
Publisher
Elsevier
Publication Date
Volume
95
Issue
1
Identifiers
DOI: 10.1016/0005-2787(65)90204-2

Abstract

Abstract Thin-layer chromatographic separations on a new layer, microcrystalline cellulose “Avicel”, and on DEAE-cellulose are presented. They include: 1. 1. R F values of 15 bases and methylated analogues of the bases in six different solvents, of 11 nucleosides in 5 different solvents, and of 8 nucleotides in two solvents. 2. 2. Separation of mixtures of deoxynucleoside and ribonucleoside, mono-, di-, and tri-phosphates of the adenine, guanine, uracil, and cytosine series. 3. 3. Separation of mixtures of the four ribonucleoside 2′,3′-monophosphates, of the four ribonucleosides 5′-monophosphates, and of the four deoxyribonucleoside 5′-monophosphates. The advantages of microcrystalline cellulose over other materials for thin-layer chromatography are discussed.

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