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Cell physiology and metabolic flux response ofKlebsiella pneumoniaeto aerobic conditions

Authors
Journal
Process Biochemistry
1359-5113
Publisher
Elsevier
Publication Date
Volume
44
Issue
8
Identifiers
DOI: 10.1016/j.procbio.2009.04.004
Keywords
  • Klebsiella Pneumoniae
  • 1
  • 3-Propanediol
  • Metabolic Flux Analysis
  • Cell Physiology
  • Aerobic Fermentation
  • Aeration Control
Disciplines
  • Biology

Abstract

Abstract Cell physiology and metabolic flux distribution of Klebsiella pneumoniae under anaerobic, micro-aerobic and sufficient aerobic conditions were compared. Comparing with the anaerobic condition, the carbon flux flowed from glycerol to biomass increased 10.1% and 389.9%, while the flux flowed to 1,3-propanediol decreased 10.3% and 92.9% under micro-aerobic and sufficient aerobic conditions, respectively. Furthermore, the carbon flux flowed to TCA cycle increased 5.9% and 31.0% under such two conditions. The energy analysis results revealed that the oxygen was favorable for the NADH 2 synthesis, but excessive oxygen was disadvantage for the NADH 2 utilization in 1,3-propanediol synthesis process. So, the aeration control is significant for the aerobic 1,3-propanediol fermentation. This work is considered helpful for the further understanding of the glycerol metabolism by Klebsiella pneumoniae under aerobic condition and to establish a rational aeration control strategy for 1,3-propanediol aerobic fermentation in a large-scale bioreactor.

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