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Mutants That Produce Nitrogenase in the Presence of Ammonia

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  • Biological Sciences: Microbiology


Mutants of Azotobacter vinelandii that fix N2 in the presence of excess NH4+ have been isolated. A mutant that was unable to synthesize component I and component II of nitrogenase was spontaneously reverted to the N2-fixing phenotype. Of 21 revertants picked, 7 revertants were not as sensitive as the wild type to repression. A derepressed mutant is as sensitive as the wild type to growth inhibition by 2-methylalanine in the presence of glucose.

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