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Isolation of ribonucleoprotein particles from rat brain microsomes by a liquid two-phase system

Authors
Journal
Journal of Ultrastructure Research
0022-5320
Publisher
Elsevier
Publication Date
Volume
2
Issue
3
Identifiers
DOI: 10.1016/s0022-5320(59)80008-3
Disciplines
  • Chemistry

Abstract

The particle component of rat brain microsomes was separated from the membrane structures by partition in a two-phase aqueous system of dextran and methylcellulose. The particles behaved as could be expected from their observed size. After the separation, electron microscopy showed a high concentration of particles almost without membranes, and a parallel increase in the concentration of RNA was demonstrated by chemical analysis.

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