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Photosynthetic Gene Expression in Emiliania huxleyi by qualtitative RT-PCR Analysis

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8 Alfred-Wegener-Institute For Polar and Marine Research Bremerhaven Photosynthetic Gene Expression in Emiliania huxleyi By quantitative- RT PCR analysis ___________________________________________________________________________ Master Thesis Work by Umesh Gangishetti Fachbereich 2 Dept of Biology and Chemistry University of Bremen Bremerhaven 2005-2006 2 Declaration This is in accordance with the Masters Thesis test order for the course of studies Molecular biology and biochemistry at the University of Bremen. I hereby certify that the facts available in the present thesis was independently written and used no different than the sources and aids indicated by me. The used sources literally or content-wise inferred places are marked accordingly. Bremerhaven, the 18.7.2005 Umesh Gangishetti 3 Dedicated to my loving Mother and my Family Members 4 The real study say the Upanishads, is not the study of themselves but study of that” by which we realize the changeless”. Upanishads 5 Abbreviation Listing bp Base pair cDNA Complementary deoxyribonucleic acid CHP Conservative hypothetical protein DEPC Diethylpyrocarbonate DMS Dimethylsulfide DMSP Dimethylsufoniopropionate DNA Deoxyribonucleic acid DNase Deoxyribonuclease dNTP Deoxynucleotide triphosphate EDTA Ethylendiamin-tetra acetic acid kb kilo-basepair mRNA Messenger RNA OD Optical Density oligo-dT oligo- deoxythymidine PCR Polymerase chain reaction PL-tubes Phase Lock tubes PS I Photosystem I PSII Photosystem II qRT quantitative reverse transcription RNA Ribonucleic acid RNAse Rib nuclease Rpm

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