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F9 teratocarcinoma aggregates express villin upon differentiation into visceral endoderm-like cells

Authors
Journal
Biology of the Cell
0248-4900
Publisher
Wiley Blackwell (Blackwell Publishing)
Publication Date
Keywords
  • F9 Teratocarcinoma
  • Visceral Endoderm Differentiation
  • Mouse Villin
  • Actin Binding Protein
  • Afp
Disciplines
  • Biology

Abstract

Abstract The visceral endoderm of the mouse embryo is a polarized epithelium which has recently been shown to express villin, a major actin binding component of absorptive epitheliums. We report here that villin is induced during differentiation of aggregates of the mouse embryonal carcinoma F9, an in vitro system widely used to study extraembryonic endoderm differentiation. Identical results were obtained with a variant of F9 which carries an immortalizing vector. Villin is coexpressed with F-actin and with α-foetoprotein, in most of the visceral endoderm-like cells lining the aggregates. This system is potentially useful to study (i) the induction of villin expression and (ii) the establishment of polarity in the visceral endoderm epithelium.

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