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Recombinant streptavidin-C3bot for delivery of proteins into macrophages

Authors
Journal
Toxicon
0041-0101
Publisher
Elsevier
Publication Date
Volume
75
Identifiers
DOI: 10.1016/j.toxicon.2013.02.002
Keywords
  • Clostridium Botulinum C3 Transferase
  • Fusion Protein
  • Streptavidin
  • Biotin
  • Cellular Transport
  • Macrophages
Disciplines
  • Biology
  • Pharmacology

Abstract

Abstract We demonstrated previously that monocytes and macrophages are target cells for the Rho-modifying Clostridium botulinum C3 ADP-ribosyltransferase. Here, we report the construction, expression and characterization of a recombinant streptavidin-C3 fusion protein which allows for delivery of biotin-labelled molecules into the cytosol of macrophages via enzymatically inactive C3bot1E174Q. The enzyme domain of diphtheria toxin was used as cargo to demonstrate proof of principle. This transport system could represent an attractive tool for experimental monocyte/macrophage pharmacology.

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