Affordable Access

Publisher Website

Protein biosynthesis in trypanosomidae—I.in vivoincorporation of leucine-C14inTrypanosoma cruzi

Authors
Journal
Comparative Biochemistry and Physiology
0010-406X
Publisher
Elsevier
Publication Date
Volume
18
Issue
2
Identifiers
DOI: 10.1016/0010-406x(66)90183-6
Disciplines
  • Biology

Abstract

Abstract 1. 1. Conditions for the in vivo incorporation of dl-leucine-1-C 14 into the proteins of Trypanosoma cruzi are reported in this paper. Best results were obtained with KC1 0·154 M as suspension medium as compared to 0·25 M sucrose. 2. 2. Optimal pH in the absence of glucose was 7·6, while in its presence was 8·0. 3. 3. The addition of glucose increases the incorporation of the isotope into total proteins, while various metabolites of the Krebs cycle have an opposite effect. 4. 4. Cell fractionation studies indicate a rapid labeling of microsomal protein. Chromatography and radioautography show that isoleucine and valine are also labeled in the protein hydrolysate. 5. 5. Our results suggest that an active mechanism, similar to those reported for higher organisms, might be involved in protein biosynthesis in T. cruzi.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Protein biosynthesis in Trypanosomidae. I. In vivo...

on Comparative biochemistry and p... June 1966

Protein biosynthesis in trypanosomidae. II. The me...

on Experimental Parasitology October 1967

Protein biosynthesis in trypanosomidae:II. The met...

on Experimental Parasitology Jan 01, 1967

Upregulation of sterol C14-demethylase expression...

on Molecular and Biochemical Para... Jan 01, 2005
More articles like this..