Affordable Access

Publisher Website

Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marineVibrio alginolyticus

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
422
Issue
2
Identifiers
DOI: 10.1016/s0014-5793(98)00016-7
Keywords
  • Na+-Translocating Nadh-Quinone Reductase
  • Respiratory Chain
  • Na+Pump
  • Subunit Structure
  • Vibrio Alginolyticus
Disciplines
  • Biology

Abstract

Abstract We previously reported that the purified Na +-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus is composed of three major subunits, α, β and γ. NQR operon was sequenced and was found to be composed of 6 structural genes. Among these genes, nqr1, nqr3 and nqr6 were identified to code for α-, γ- and β-subunits, respectively. The protein products from nqr2, nqr4 and nqr5, however, were not reported. The sequence data predicted that these three proteins are very hydrophobic and may be unusual in mobility and staining on SDS-PAGE. By modifying the detection method of proteins on SDS-PAGE, we could detect all six subunits encoded by NQR operon in the purified NQR complex. The open reading frame of each subunit was identified from its N-terminal amino acid sequence.

There are no comments yet on this publication. Be the first to share your thoughts.