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CAP5.5, a life-cycle-regulated, cytoskeleton-associated protein is a member of a novel family of calpain-related proteins inTrypanosoma brucei

Authors
Journal
Molecular and Biochemical Parasitology
0166-6851
Publisher
Elsevier
Publication Date
Volume
116
Issue
1
Identifiers
DOI: 10.1016/s0166-6851(01)00296-1
Keywords
  • Acylation
  • Calpains
  • Cytoskeleton
  • Differentiation
  • Protease
  • Trypanosome
Disciplines
  • Biology
  • Computer Science

Abstract

Abstract The cell shape of African trypanosomes is determined by the presence of an extensive subpellicular microtubule cytoskeleton. Other possible functions of the cytoskeleton, such as providing a potential framework for signalling proteins transducing information from the intracellular and extracellular environment, have not yet been investigated in trypanosomes. In this study, we have identified a novel cytoskeleton-associated protein in Trypanosoma brucei. CAP5.5 is the first member of a new family of proteins in trypanosomes, characterised by their similarity to the catalytic region of calpain-type proteases. CAP5.5 is only expressed in procyclic, but not in bloodstream, trypanosomes. Furthermore, CAP5.5 has been shown to be both myristoylated and palmitoylated, suggesting a stable interaction with the cell membrane. A bioinformatics analysis of the trypanosome genome revealed a diverse family of calpain-related proteins with primary structures similar to CAP5.5, but of varying length. We suggest a nomenclature for this new family of proteins in T. brucei.

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