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Brugia malayi:Stage-specific expression of carbohydrates containingN-acetyl-d-glucosamine on the sheathed surfaces of microfilariae

Authors
Journal
Experimental Parasitology
0014-4894
Publisher
Elsevier
Publication Date
Volume
58
Issue
2
Identifiers
DOI: 10.1016/0014-4894(84)90033-x
Keywords
  • Brugia Malayi
  • Nematode Parasitic
  • Filariae
  • Surface
  • Sheath
  • Lectin
  • Carbohydrate
  • N-Acetyl-D-Glucosamine

Abstract

Abstract Microfilariae, infective larvae, and adult worms of Brugia malayi were incubated with a panel of seven lectins in order to study the expression of surface carbohydrates. Infective larvae and adult worms did not bind any of the lectins utilized. Microfilariae, on the other hand, bound wheat germ agglutinin. The binding of this lectin was saturable and specific, and attributed to the presence of N-acetyl- d-glucosamine. In addition, microfilariae derived in vitro bound concanavalin A, indicating the presence of glucose and/or mannose on this stage of the parasite. The fact that similar concanavalin A binding was not seen on microfilariae recovered directly from the infected host implies that there is masking or loss of parasite surface antigens as microfilariae mature in vivo.

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