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Quantum dots in axillary lymph node mapping: Biodistribution study in healthy mice

BMC Cancer
Springer (Biomed Central Ltd.)
Publication Date
DOI: 10.1186/1471-2407-8-111
  • Research Article
  • Physics

Abstract ral ss BioMed CentBMC Cancer Open AcceResearch article Quantum dots in axillary lymph node mapping: Biodistribution study in healthy mice Anne Robe, Emilie Pic, Henri-Pierre Lassalle, Lina Bezdetnaya, François Guillemin and Frédéric Marchal* Address: CRAN, Nancy-University, CNRS, Centre Alexis Vautrin, Avenue de Bourgogne, 54511 Vandoeuvre-lès-Nancy Cedex, France Email: Anne Robe - [email protected]; Emilie Pic - [email protected]; Henri-Pierre Lassalle - [email protected]; Lina Bezdetnaya - [email protected]; François Guillemin - [email protected]; Frédéric Marchal* - [email protected] * Corresponding author Abstract Background: Breast cancer is the first cause of cancer death among women and its incidence doubled in the last two decades. Several approaches for the treatment of these cancers have been developed. The axillary lymph node dissection (ALND) leads to numerous morbidity complications and is now advantageously replaced by the dissection and the biopsy of the sentinel lymph node. Although this approach has strong advantages, it has its own limitations which are manipulation of radioactive products and possible anaphylactic reactions to the dye. As recently proposed, these limitations could in principle be by-passed if semiconductor nanoparticles (quantum dots or QDs) were used as fluorescent contrast agents for the in vivo imaging of SLN. QDs are fluorescent nanoparticles with unique optical properties like strong resistance to photobleaching, size dependent emission wavelength, large molar extinction coefficient, and good quantum yield. Methods: CdSe/ZnS core/shell QDs emitting around 655 nm were used in our studies. 20 µL of 1 µM (20 pmol) QDs solution were injected subcutaneously in the anterior paw of healthy nude mice and the axillary lymph node (ALN) was identified visually after injection of a blue dye. In vivo fluorescence spectroscopy was performed on ALN before the mice were sacrificed

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