Keladi tikus root (Typhonium flagelliforme (Lodd) BI) is one of plants that is used for cancer healing. The plant contents flavonoid, tannin, terpenoid and steroid. Flavonoid and terpenoid compound groups are known for anti cancer activities. The investigation is conducted to test cytotoxic effect of ethanol free dry extract of keladi tikuroot (Typhonium jlagelliforme (Lood)BI) against brest cancer cell MCF-7. Maceration extraction method used 50% ethanol solvent and vaporized until ethanol.free dry extract of keladi tikus root was obtained. The test used 5 level of concentration, those were 50, 75, I 00, 125 and 150 µg/ml with 3 times iteration. DMSO was used as negative control and Cisplatin with concentration of 4, 6, 8, 19, 12 µg/ml were used as positive control. Test result showed ethanol free dry extract of keladi tikus root has value of LC50 = 89,15 and Cisplatin has value of LC50 = 7,84 µg/ml. Further investigation of advance sitotoxic test value LC50 and value LC50 against fraction of 50% ethanol extract is necessary to obtain active compound against brest cancer cell MCF-7. Ethanol Extract 50% of keladi tikus root (Typhonium flagelliforme (Lood) BI), Breast Cancer, MCF-7 Cell, LC50.