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Dosing-time dependent oxidative effects of sodium nitroprusside in brain, kidney, and liver of mice

Authors
Journal
Environmental Toxicology and Pharmacology
1382-6689
Publisher
Elsevier
Volume
38
Issue
2
Identifiers
DOI: 10.1016/j.etap.2014.08.013
Keywords
  • Malondialdehyde
  • Sodium Nitroprusside
  • Mice
  • Brain
  • Kidney
  • Liver

Abstract

Abstract The purpose of this study was to investigate if the oxidative effects of sodium nitroprusside (SNP) are dosing-time dependent. Therefore, the variation of malondialdehyde (MDA) status was assessed after a single i.p. administration of SNP (2.5mgkg−1 b.w.) or vehicle (9‰ NaCl) to different and comparable groups of mice (n=48) at two different circadian times (1 and 13h after light onset [HALO]). Brain, kidney, and liver tissues were excised over 36h, and their MDA contents were estimated at 0, 1, 3, 6, 9, 12, 24, and 36h after SNP administration. Results indicated mean MDA level was not significantly changed in each investigated tissue compared with the control. In contrast, the mean MDA value varied among organs and was comparable in brain and liver but lower than in kidney. The data show SNP significantly (P<0.05) increases MDA status in both tissues and exerts time-dependent oxidative effects with the greatest toxicity coinciding with the beginning of the diurnal rest span (local time: 08:00h, i.e., at 1 HALO). The obtained results reveal SNP-induced oxidative damage (evidenced by MDA accumulation) varies according to both the dosing-time and the target organ.

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