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Purification and characterization of the N- and C-terminal cyanogen bromide peptide of salt-soluble type III collagen

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
80
Issue
1
Identifiers
DOI: 10.1016/0003-2697(77)90646-7
Disciplines
  • Biology
  • Pharmacology

Abstract

Abstract Fetal calf skin was extracted with neutral buffer in the presence of enzyme inhibitors. Type III collagen was isolated from the extract and cleaved with cyanogen bromide. The N- and C-terminal cyanogen bromide peptides were purified and characterized. The sizes and amino acid compositions of the N- and C-terminal non-triple-helical extensions of type III collagen were deduced.

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